《植物生理学报》 2018, 54(7): 1179-1185

生物技术在青蒿素合成中的应用

李姣^#, 霍晋彦^#, 于宗霞^*, 冯宝民^*

大连大学生命科学与技术学院, 辽宁大连116622

通信作者：于宗霞；E-mail: yuzongxia@sibs.ac.cn, fbmdlu@163.com

摘　要：

青蒿素是迄今为止治疗疟疾的有效成分, 主要从青蒿(黄花蒿)中提取获得。然而黄花蒿中青蒿素的含量很低, 难以满足临床用药需求。本文归纳并总结了近年来用于提高青蒿素产量的生物技术方法, 包括提高野生青蒿中青蒿素产量、使用转基因手段提高青蒿素产量以及青蒿素在其他生物中合成等方面的研究进展, 为青蒿素合成相关研究提供理论依据, 开拓研究思路。

关键词：青蒿素合成; 生物技术; 转基因技术; 生物合成

收稿：2018-04-20   修定：2018-06-29

资助：国家自然科学基金(31700267、31270398和31540006)、植物分子遗传国家重点实验室、辽宁省博士科研启动基金(201501195)和大连市青年科技之星(2017RQ025)。

Application of biotechnology in the biosynthesis of artemisinin

LI Jiao^#, HUO Jin-Yan^#, YU Zong-Xia^*, FENG Bao-Min^*

College of Life Sciences and Biotechnology, Dalian University, Dalian, Liaoning 116622, China

Corresponding author: YU Zong-Xia; E-mail: yuzongxia@sibs.ac.cn, fbmdlu@163.com

Abstract:

Artemisinin is the most effective component in malaria treatment so far. It is mainly extracted from Artemisia annua. However, the content of artemisinin in A. annua is very low which makes it difficult to meet the needs of clinical medication. This article collects and summarizes the biotechnology methods used to increase artemisinin production in recent years, including the use of biotechnology to increase the production of artemisinin in wild A. annua, the use of transgenic methods to increase artemisinin content, and the use of genetic engineering to re-construct artemisinin biosynthesis pathway in other organisms, so as to provide a theoretical basis and ideas for research on artemisinin biosynthesis.

Key words: artemisinin synthesis; biotechnology; genetically modified technology; biosynthesis